The morphogenetic capability and the viability of regenerants in micropropagated orchid hybrids infected with viral pathogens
Teresa Cybularz-Urban, Ewa Hanus-Fajerska
Department of Botany
Faculty of Horticulture, University of Agriculture in Kraków
29 Listopada 54, 31-425 Kraków, Poland
e-mail: email@example.com, firstname.lastname@example.org
The micropropagation efficiency of four interspecific Cattleya hybrids (clones: 69,
75, 149 and 150) infected with Cymbidium mosaic (CyMV) and Odontoglossum
ringspot (ORSV) viruses was assessed. The aim of experiments was to evaluate
with that model to what extent viral infection affects the morphogenesis in vitro in
orchid hybrids of different origin. The effectiveness of plant material exposure to
therapeutic levels of plant growth regulators supplied with media in order to
suppress infection was also verified. The vitality of proliferating infected shoot
cultures was limited, and the symptoms of senility were frequently observed.
Regardless genotype of the studied clone, during acclimation to ex vitro conditions
considerable losses become visible what indicates the necessity of testing the donor
material for possible latent viral infections. Infection with CyMV and ORSV
mostly persisted in every tested clone.
Cybularz-Urban T., Hanus-Fajerska E., 2008. The
morphogenetic capability and the viability of regenerants in
micropropagated orchid hybrids infected with viral pathogens.
Folia Horticulturae 20/2: 93-102.