Optimization of isolation conditions of Cymbidium protoplasts
Department of Botany
Faculty of Horticulture, Agricultural University in Kraków
29 Listopada 54, 31-425 Kraków, Poland
Several factors influencing high-efficiency method of protoplast isolation from
Cymbidium, including kind of explants (primary or secondary), enzymes
(quantitative and qualitative), time of incubation, properties between enzymatic
mixture and weight of explants, as well as the composition of protoplast cultivation
media were investigated. The best isolation efficiency (1.1 × 107) was obtained
from column. Modified Tan et al. (1987) protoplast cultivation medium with
5.4 μM NAA and 2.3 μM ZEAT turned out to be the most suitable for protoplasts.
Several-cells aggregates were obtained on this medium.
Pindel A., 2007. Optimization of isolation conditions of Cymbidium
protoplasts. Folia Horticulturae 19/2: 79-88.