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Optimization of isolation conditions of Cymbidium protoplasts

Anna Pindel

Department of Botany
Faculty of Horticulture, Agricultural University in Kraków
29 Listopada 54, 31-425 Kraków, Poland
e-mail: apindel@ogr.ar.krakow.pl

Several factors influencing high-efficiency method of protoplast isolation from Cymbidium, including kind of explants (primary or secondary), enzymes (quantitative and qualitative), time of incubation, properties between enzymatic mixture and weight of explants, as well as the composition of protoplast cultivation media were investigated. The best isolation efficiency (1.1 × 107) was obtained from column. Modified Tan et al. (1987) protoplast cultivation medium with 5.4 μM NAA and 2.3 μM ZEAT turned out to be the most suitable for protoplasts. Several-cells aggregates were obtained on this medium.

Pindel A., 2007. Optimization of isolation conditions of Cymbidium protoplasts. Folia Horticulturae 19/2: 79-88.