RAPD technique application for intraline evaluation of androgenic carrot plants
Mirosława Staniaszek, Hanna Habdas
Department of Genetics, Breeding and Biotechnology
Research Institute of Vegetable Crops
Konstytucji 3 Maja 1/3, 96-100 Skierniewice, Poland
The aim of this investigation was the evaluation of intraline uniformity of androgenic carrot plants,
obtained by the use of anther culture technique. The material studied comprised plants of 30 lines/clones
of R1 generation. The evaluation was carried out using 10 RAPD primers:
OPA02, OPA04, OPA07, OPA11, OPB04, OPB11, OPB12, OPB15, OPB17 and OPB18. The RAPD analysis demonstrated
significant plant variation within each of the lines studied. This was demonstrated by the presence of quantitative
differences in the amplification profiles obtained for individual plants within each line analysed. The results
obtained showed that the lines tested were not homozygous and were therefore not DH lines.
Staniaszek M., Habdas H., 2006. RAPD technique application for intraline evaluation of androgenic carrot plants.
Folia Horticulturae 18/2: 87-97.