Regeneration ability of petiole, leaf and petal explants in gerbera cut flower cultures in vitro
Surinder Kumar, Jitender Kumar Kanwar
Department of Biotechnology
University of Horticulture & Forestry
Solan 173 230 (H.P.), India
The maximum callus induction and growth in petal explant was observed on MS medium supplemented
with 1, 1.5 and 2 mg l-1 2,4-D and in leaf with 1.5 and
2 mg l-1 2,4-D, however the callus failed to differentiate into shoots.
Petiole explants did not respond to any of the treatments tried. The calli derived from leaf explant
differentiated into roots with NAA in the medium. About 53% of the calli produced from petal explant
developed five shoots per callus when transferred to shoot regeneration medium containing
2 mg l-1 BA and 0.5 mg l-1 IAA. The in vitro
shoots were rooted by quick dipping the basal portion of the shoot in 2000 mg l-1 IBA
solution. 50-60% survival of the plants was observed after 30 days of transfer to pots.
Kumar S., Kanwar J.K., 2006. Regeneration ability of petiole, leaf and petal explants in gerbera cut flower
cultures in vitro. Folia Horticulturae 18/2: 57-64.