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Regeneration ability of petiole, leaf and petal explants in gerbera cut flower cultures in vitro

Surinder Kumar, Jitender Kumar Kanwar

Department of Biotechnology
University of Horticulture & Forestry
Solan 173 230 (H.P.), India
e-mail: skuhf@yahoo.com

The maximum callus induction and growth in petal explant was observed on MS medium supplemented with 1, 1.5 and 2 mg l-1 2,4-D and in leaf with 1.5 and 2 mg l-1 2,4-D, however the callus failed to differentiate into shoots. Petiole explants did not respond to any of the treatments tried. The calli derived from leaf explant differentiated into roots with NAA in the medium. About 53% of the calli produced from petal explant developed five shoots per callus when transferred to shoot regeneration medium containing 2 mg l-1 BA and 0.5 mg l-1 IAA. The in vitro shoots were rooted by quick dipping the basal portion of the shoot in 2000 mg l-1 IBA solution. 50-60% survival of the plants was observed after 30 days of transfer to pots.

Kumar S., Kanwar J.K., 2006. Regeneration ability of petiole, leaf and petal explants in gerbera cut flower cultures in vitro. Folia Horticulturae 18/2: 57-64.