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Growth in vitro cultures of strawberry (Fragaria × ananassa Duch.) depending on different photoperiods

Wojciech Litwińczuk, Agnieszka Zubel

Department of Plant Production
Faculty of Biology and Agriculture, Rzeszów University
M. Ćwiklińskiej 2, 35-601 Rzeszów, Poland
e-mail: wlitw@univ.rzeszow.pl

The influence of three photoperiods: a) 16/8 (d/n) - control, b) 4/2 (d/n) (4 cycles per 24 h), and c) 22/2 (d/n) on the growth of in vitro cultures of strawberry (Fragaria × ananassa Duch.) cultivars ‘Senga Sengana’ and ‘Elsanta’ was investigated. The application of 22/2 (d/n) photoperiod was not advantageous as it did not increase culture efficiency, stimulated the growth of callus at the explant base and caused chlorosis of leaves. Photoperiod 4/2 (d/n) significantly enhanced shoot proliferation of ‘Senga Sengana’ cultures when compared to control 16/8 (d/n). The reaction of ‘Elsanta’ cultures was not so distinct. As the change of photoperiod from 16/8 (d/n) to 4/2 (d/n) needs neither special instrumentation nor creates any additional costs it could be recommended in strawberry micropropagation, especially in the case of cultivar ‘Senga Sengana’.

Litwińczuk W., Zubel A., 2005. Growth in vitro cultures of strawberry (Fragaria × ananassa Duch.) depending on different photoperiods. Folia Horticulturae 17/2: 81-87.