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Initiation of in vitro cultures of chosen endangered European species of orchids

Anna Pindel*, Zbigniew Pindel**

**Department of Botany
**Department of Ornamental Plants
**Faculty of Horticulture, Agricultural University in Kraków
**29 Listopada 54, 31-425 Kraków, Poland
**e-mail: apindel@ogr.ar.krakow.pl, ropindel@cyf-kr.edu.pl

The effect of the kind of explants and the composition of media on the initial stages of micropropagation of five orchid species (Cypripedium calceolus, Dactylorhiza maculata, Epipactis helleborine, Goodyera repens, and Gymnadenia conopsea) was investigated. The sections of green capsules, and young ovaries were found to be suitable for this purpose. Depending on the species explants produced protocorm-like bodies, protocorms with occasional root- and shoot-primordia, or callus - on the MS medium with additions of 0.3 mg l-1 NAA, 1.0 mg l-1 BA, and 1% AC.

Pindel A., Pindel Z., 2004. Initiation of in vitro cultures of chosen endangered European species of orchids. Folia Horticulturae 16/2: 111-117.