Folia Horticulturae

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The effect of the growing condition on the growth and reproduction of tulip bulbs produced in vitro

Małgorzata Podwyszyńska*, Jacek S. Nowak**

**Department of Physiology and Morphogenesis of Ornamental Plants
**Department of Cultivation and Fertilization of Ornamental Plants
**Research Institute of Pomology and Floriculture
**Pomologiczna 18, 96-100 Skierniewice, Poland
**e-mail: mpodwysz@insad.pl

The aim of the study was the shortening of the period needed to obtain flowering tulip plants derived from micropropagation. Bulbs of the tulip ‘Prominence’ and ‘Blue Parrot’ produced in vitro were rooted for three months in the peat growing substrates in the dark at 9°C. Then, plants were grown in a growth chamber at 15°C under light (80-100 μmol m-2 s-1) or in a greenhouse and fertilized every two weeks with Peters Professional 20-10-20 or calcium nitrate, both at the concentration of 0.05 or 0.1%. Bulbs were also grown in a field. It was shown that both in the growth chamber and the greenhouse the yields of daughter bulbs were poor. The daughter bulbs produced in the growth chamber were 20-40% smaller than the mother ones, with an exception of combination in which the 0.05% Peters Professional was used, where the daughter bulbs were 13.5% larger than the mother ones. In the greenhouse, the daughter bulbs larger than the mother ones by 10-40% were noted at Peters Professional 20-10-20 fertilization. The field conditions, with the mild winters in both seasons 2000/2001 and 2001/2002, had highly beneficial effect on the efficiency of bulb reproduction. The daughter bulbs were 2-5 times larger than the mother bulbs. It is supposed that the factor limiting the growth of bulbs in the growth chamber was too low quantum irradiance and in the greenhouse too high temperature.

Podwyszyńska M., Nowak J.S., 2004. The effect of the growing condition on the growth and reproduction of tulip bulbs produced in vitro. Folia Horticulturae 16/1: 133-145.