Folia Horticulturae

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Microbiological analysis of air in the in vitro cultures laboratories

Ewa Reby*, Maria Kowalik**

**Department of Genetics, Plant Breeding and Seed Science
**Department of Plant Protection
**Faculty of Horticulture, University of Agriculture in Kraków
**29 Listopada 54, 31-425 Kraków, Poland
**e-mail: ereby@poczta.onet.pl, mkowalik@bratek.ogr.ar.krakow.pl

The analyses of air were carried out in scientific and commercial laboratories of in vitro cultures, in spring and autumn. The quantitative and qualitative composition of micro-organisms found in the air of the laboratories depended on the type of laboratory, place of sampling, and the time of analysis. The most often discovered were bacteria belonging to: Micrococcus / Sarcina / Staphylococcus and fungi: Alternaria, Aspergillus, Cladosporium, Paecilomyces, Penicillium, and Trichoderma. The results gave a basis for the conclusion that bacteria and fungal propagules present in the air of the laboratories are a source of the infections of plant material and media.

Reby E., Kowalik M., 2003. Microbiological analysis of air in the in vitro cultures laboratories. Folia Horticulturae 15/2: 211-216.