Microbiological analysis of air in the in vitro cultures laboratories
Ewa Reby*, Maria Kowalik**
**Department of Genetics, Plant Breeding and Seed Science
**Department of Plant Protection
**Faculty of Horticulture, University of Agriculture in Kraków
**29 Listopada 54, 31-425 Kraków, Poland
The analyses of air were carried out in scientific and commercial laboratories of in vitro cultures,
in spring and autumn. The quantitative and qualitative composition of micro-organisms found in the air of the
laboratories depended on the type of laboratory, place of sampling, and the time of analysis. The most often
discovered were bacteria belonging to: Micrococcus / Sarcina / Staphylococcus and fungi:
Alternaria, Aspergillus, Cladosporium, Paecilomyces, Penicillium, and
Trichoderma. The results gave a basis for the conclusion that bacteria and fungal propagules present
in the air of the laboratories are a source of the infections of plant material and media.
Reby E., Kowalik M., 2003. Microbiological analysis of air in the in vitro cultures laboratories. Folia
Horticulturae 15/2: 211-216.