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Anther cultures of carrot (Daucus carota L.)

Adela Adamus, Barbara Michalik

Department of Genetics, Plant Breeding and Seed Science
Faculty of Horticulture, University of Agriculture in Kraków
29 Listopada 54, 31-425 Kraków, Poland
e-mail: kghn@ogr.ar.krakow.pl

The research aimed at the application of androgenesis to produce carrot doubled haploids. Twenty-one genetically different carrot stocks were used. Donor plants were grown in the greenhouse, growth chamber, and in the field. Five media were used for anther cultures. Three years of research showed that callus was formed in frequency of 0 to 21% and embryos 0 to 1.6%, depending on the genotype, induction medium composition, and conditions of donor plant growth. Anthers from plants of ‘Koral’ were the most responsive and produced callus with the frequency of 20.8% and embryos - 1.2%. MS medium supplemented with 1.0 mg dm-3 2,4-D and 20 g dm-3 sucrose was the most effective in the induction of anthers response. Better results were observed when donor plants were grown in the greenhouse or in the growth chamber in comparison with those from the field. Plants were regenerated from callus and embryos. Ploidy level analysis of the obtained regenerants revealed the presence of haploids (3%), diploids (86%), and polyploids (11%). Among the diploid plants only 1% regenerants resulted from androgenesis, this being proved by the Pgi isozyme test.

Adamus A., Michalik B., 2003. Anther cultures of carrot (Daucus carota L.). Folia Horticulturae 15/2: 49-58.