Folia Horticulturae

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In vitro propagation of protected species Gentiana pneumonanthe L. for ornamental horticultural use

Bożena Pawłowska, Anna Bach

Department of Ornamental Plants
Faculty of Horticulture, University of Agriculture in Kraków
29 Listopada 54, 31-425 Kraków, Poland
e-mail: ropawlow@cyf-kr.edu.pl

The micropropagation method for endangered species Gentiana pneumonanthe L. was developed from mature and immature seeds (in capsules). As explants for organogenesis shoot tips and one-nodal cuttings of seedlings grown in vitro were used. Four cytokinins (BA, kinetin, thidiazuron, 2iP) and additionally IAA and gibberellin at the concentration of 1.5 μM were tested in the experiments. The highest multiplication rate was achieved in the culture of the one-nodal cuttings on medium supplemented with 10.0 μM BA. Excised axillary shoots were rooted successfully on the medium with IAA, NAA, and IBA auxins at a concentration of 0.5 and 1 μM while the best effect was found in the case of IAA. Shoots formed roots also on a medium without growth regulators. Rooted plantlets were potted in soil (65% survival) and after acclimatisation in a greenhouse were successfully planted outdoors in field conditions. The plants had a greater number of flowers and stems than plants grown in a natural habitat. Plants obtained in vitro are valuable as ornamental perennials and can be used in the active protection of this species endangered with extinction.

Pawłowska B., Bach A., 2003. In vitro propagation of protected species Gentiana pneumonanthe L. for ornamental horticultural use. Folia Horticulturae 15/1: 113-122.