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Studies of protoplast culture from callus of Asparagus densiflorus ‘Sprengeri’

Anna Pindel

Department of Botany
Faculty of Horticulture, University of Agriculture in Kraków
29 Listopada 54, 31-425 Kraków, Poland
e-mail: ropindel@cyf-kr.edu.pl

In the first phase the possibility of obtaining the callus tissue with the structure that facilitates the isolation of viable protoplasts of Asparagus densiflorus ‘Sprengeri’ was investigated. Then the composition of the enzymatic mixture and the incubation conditions necessary to obtain an efficient method of protoplast isolation was elaborated. The high efficiency (9×107 protoplasts g-1 fresh weight of callus from MS medium with 10.0 mg l-1 dicamba) was guaranteed by the composition: 1.2% cellulysin + 0.3% macerozyme + 1.0% rhozyme in 7% sorbitol with 0.1% MES for 20 h. In the final phase of the investigations a few compositions of media were tested. The best plating efficiency was obtained on MS medium with additions of 0.5 mg l-1 zeatin, 1.0 mg l-1 NAA, 0.2 mg l-1 2,4-D, and 5 ml coconut milk.

Pindel A., 2002. Studies of protoplast culture from callus of Asparagus densiflorus ‘Sprengeri’. Folia Horticulturae 14/2: 243-250.