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Tissue culture propagation of Eucalyptus gunnii Hook.

Zbigniew Koziara

Department of Ornamental Plants
Faculty of Horticulture, University of Agriculture in Kraków
29 Listopada 54, 31-425 Kraków, Poland

The investigation on the in vitro propagation of Eucalyptus gunnii was composed of three stages. Stage one concerned the effects of stratification, composition of media, and four methods of seed disinfection (with "Domestos" - a household bleach, chloramine, or calcium hypochlorite) on the dynamics of germination. The two media E1 and E2 on the MS basis with an addition of 3% sucrose and solidified with 0.7% agar were used in the experiment. The E1 medium was supplemented by calcium panthothenian, inositol, and PVP at doses of 2, 100, and 100 mg dm-3 respectively, while the E2 medium was enriched with 1 mg dm-3 BA and 0.1 mg dm-3 NAA. The two media mentioned above ensured good germination of seeds in the in vitro cultures. The stratification did not affect germination. The application of "Domestos" at a ratio of 1:5 for 30 min was found to be the most efficient in disinfecting seeds for the in vitro cultures. Moreover, it improved the parameters and dynamics of germination, supplying initial planting material of very good quality.

In the second stage the value of five different media for the proliferation of Eucalyptus gunnii transplants was determined. On the grounds of literature data, the basic MS medium was supplemented with the following growth regulators: (B) - 1 mg dm-3 BA + 0.01 mg dm-3 IAA; (L) - 0.5 mg dm-3 BA + 0.01 mg dm-3 NAA; (J) - 0.2 mg dm-3 BA + 0.2 mg dm-3 NAA; (P) - 1 mg dm-3 BA; (D2) - 1.08 mg dm-3 kinetin + 0.53 mg dm-3 IAA. The results showed that the media did not effect statistically significant differences.

In the third stage three selected media were based on the impoverished MS medium. The best rooting was found on MS medium with the content of macroelements reduced to 1/4 CaCl2, vitamins, amino acids, and sucrose reduced to 1/2 and an addition of 10 mg dm-3 IAA. On this medium 90% of microplants developed the most abundant root system which in some cases reached 110 mm in total length, with a mean height of microplants of 170 mm. The obtained microplants were used as the starting material in the culture of regenerates in greenhouse production.

Koziara Z., 2000. Tissue culture propagation of Eucalyptus gunnii Hook. Folia Horticulturae 12/2: 79-91.