Folia Horticulturae

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Glutathione and ascorbate contents in broccoli and lettuce cultivars

Barbara Łata, Marzenna Przeradzka

Section of Basic Natural Science in Horticulture
Warsaw Agricultural University
Nowoursynowska 166, 02-787 Warsaw, Poland

The aim of the present research was to estimate the level of glutathione and ascorbate in 11 commercially available cultivars of lettuce and 3 cultivars of broccoli grown on podsol sandy soil, silty loam alluvial soil, and peat moss. Samples of plant material were harvested, frozen in liquid nitrogen and then kept at -85°C until used. Ascorbate content was measured using the colorimetric method. Total glutathione capacity was determined spectrophotometrically at 412 nm by the cycling method. The highest content of glutathione and ascorbate was noted in broccoli - on the average 221 nmol g-1 fresh weight and 92 mg 100 g-1 fresh weight respectively. The highest content of total glutathione in the studied cultivars of broccoli was obtained for Kermit F1. There were no differences in the content of ascorbate between tested cultivars of broccoli. The best antioxidant capacity among tested cultivars of lettuce was observed in Kobra, Marion, and Red Bowl, but it was rather small in comparison with broccoli.

Łata B., Przeradzka M., 1999. Glutathione and ascorbate contents in broccoli and lettuce cultivars. Folia Horticulturae 11/2: 13-22.