Regeneration of plum (Prunus domestica L.) cultivars Čačanska Rodna, Stanley, and Čačanska Najbolia from
in vitro leaf explants
Barbara Nowak, Kazimierz Miczyński
Department of Botany
Faculty of Horticulture, Agricultural University
29 Listopada 54, 31-425 Kraków, Poland
Regeneration of three plum cultivars - Čačanska Rodna, Stanley and
Čačanska Najbolia - from in vitro leaf explants was investigated on
media with various content of mineral compounds, TDZ and NAA, and under
different light conditions. The activity of silver nitrate was studied in
detail in the cultivar Čačanska Rodna. The obtained results show that
particular cultivars have very different requirements for regeneration
with regard both for the light conditions and the composition of media.
The process of regeneration was the most effective on the MS medium
containing a half-strength concentration of macro nutrients with the
addition of 0.2 mg/l NAA + 1.65 mg/TDZ. Addition of silver nitrate
stimulated regeneration only at comparatively low concentrations.
Nowak B., Miczyński K., 1997. Regeneration of plum (Prunus domestica L.)
cultivars Čačanska Rodna, Stanley, and Čačanska Najbolia from in vitro leaf
explants. Folia Horticulturae 9/1: 33-42.