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Phylogenic relationships in Blandfordia Smith (Blandfordiaceae) as revealed by isozyme analysis

Krystyna A. Johnson

Department of Environmental Biology and Horticulture
University of Technology
Sydney, Gore Hill, 2065, NSW, Australia
e-mail: Krystyna.Johnson@uts.edu.au

Ten isozyme systems were investigated by polyacrylamide gel electrophoresis to discriminate Blandfordia species grown under in vitro conditions. Peroxidase (PER), esterase (EST) and acid phosphatase (ACP) systems separated all four Blandfordia species. Glutamate oxolacetate transaminase (GOT) and superoxide dismutase (SOD) system, did not separate Blandfordia species well. Glutamate dehydrogenase (GDH), leucine amino peptidase (LAP), alcohol dehydrogenase (ADH) and alkaline phosphatase (LAP) isozyme systems were also investigated, but were not detected. An isolated tableland population of B. grandiflora from Gibraltar Range included in this study is believed to have evolved in isolation as a distinct group and requires further investigation.

Johnson K.A., 1996. Phylogenic relationships in Blandfordia Smith (Blandfordiaceae) as revealed by isozyme analysis. Folia Horticulturae 8/2: 107-119.