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Regeneration of Cymbidium orchids from leaf and root explants

Anna Pindel, Kazimierz Miczyński

Department of Botany
Faculty of Horticulture, Agricultural University
29 Listopada 54, 31-425 Kraków, Poland

The paper describes successful experiments on regeneration of Cymbidium from vegetative tissues. Explants were taken from basal parts of leaves or aerial root sections of a Cymbidium hybrid cultivated in vitro for more than a year. They were placed on agar supplied with a modified MS-medium with 88 mM sucrose, 11 μM adenine sulphate, 22.5 μM BA, and 5.5 μM NAA. After two months of cultivation direct regeneration of shoots was observed on ca. 86 per cent of leaf explants, whereas only callus was formed on 20 per cent of root explants. In subsequent transfers the media were additionally enriched with 55 μM ascrobic acid, and with 5 mM cysteine to counteract darkening of the agar. In such conditions, after ca. 4 months of cultivation in vitro, the leaf explants developed on average 3.2 shoots per explant, while the root explants formed at first undifferentiated, protocorm-like structures which, however later on, regenerated shoots at a higher rate than leaf explants (on average 15 shoots per explant). Rooting was obtained after transfer of regenerated shoots to the same medium supplied with 4-5 μM ancymidol or 0.4-4 μM ABA. Media with an optimal ancymidol concentration of 5 μM induced root formation usually after ca. 2 months of cultivation in all the tested plantlets. ABA was most effective at 0.4 μM concentration, at which 90 per cent of explants developed roots.

Pindel A., Miczyński K., 1996. Regeneration of Cymbidium orchids from leaf and root explants. Folia Horticulturae 8/2: 95-105.