Folia Horticulturae

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Effect of nitrogen level in the substrate on yield and alkaloid content in Catharanthus roseus L. (G) Don.

Barbara Łata, Ała Sadowska

Anticancer Plant Laboratory
Warsaw Agricultural University
Nowoursynowska 166, 02-787 Warsaw, Poland

The experiment was carried out in the Anticancer Plant Laboratory, Warsaw Agricultural University, in the years 1989-1991. The plants were grown in pots in the greenhouse. The pots (10 dm3) were filled with peat substrate, at pH 6.0-6.5. Six levels of nitrogen were tested: 50, 100, 150, 200, 300, and 450 mg N dm-3, 50% in the form of (NH4)2SO4 and 50% as Ca(NO3)2. Phosphorus and potassium content was constant - 100 mg P dm-3 and 200 mg K dm-3 in the form Ca(H2PO4)2·H2O and K2SO4. The substrate was analysed at fornightly intervals and N, P, K, salinity and pH were adjusted to the established levels. Fertilization had a significant effect on the leaf N, P, and K contentm yield of the crude material and plant height. The highest yield of raw material (leaves and roots) was obtained at the level of 200 mg N dm-3. Alkaloid content in the dry matter of leaves increased with the increasing N level. The highest yield of alkaloids was obtained at 300 mg N dm-3. The highest level of N (450 mg dm-3) reduced most of the parameters studied.

Łata B., Sadowska A., 1996. Effect of nitrogen level in the substrate on yield and alkaloid content in Catharanthus roseus L. (G) Don. Folia Horticulturae 8/2: 59-69.