Micropropagation of Asparagus densiflorus Jesson cv. Myriocladus
Department of Botany
Faculty of Horticulture, Agricultural University
29 Listopada 54, 31-425 Kraków, Poland
The effect of the kind of explants and the composition of media
on the micropropagation of Asparagus densiflorus Myriocladus was
investigated. Nodal sections and internodes of shoots of in vitro
seedlings and also discs of shoots from in vivo plants were found
to be suitable for this purpose. Depending on the composition of the media
the obtained callus tissue produced shoots and roots most intensely on the
MS medium with 5.5 μM NAA and 4.8 μM kinetin, and also with
2.9 μM IAA and 4.5 μM BA. The latter medium also stimulated
originated embryo-like meristematic structures. 90% regenerated plantlets
with a well-developed root system were obtained in the case of media with
ancymidol (5 μM) and an increased content of sucrose (176 mM).
Pindel A., 1995. Micropropagation of Asparagus densiflorus Jesson cv.
Myriocladus. Folia Horticulturae 7/2: 83-91.