Folia Horticulturae

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Effect of type of carbohydrates in regeneration of Hyacinthus orientalis L. in long-term cultures

Anna Bach, Bożena Pawłowska

Department of Ornamental Plants
Faculty of Horticulturae, Agricultural University
29 Listopada 54, 31-425 Kraków, Poland

The effects of glucose, sucrose, and fructose on regeneration of the plantlets on leaf explants of the Hyacinthus orientalis cultivar Delft's Blue in long-term (2-year-cycle) cultures were compared. The explants were excised both from cultured shoots chilled at 4°C for 10 weeks and from shoots incubated at 23°C, and then cultivated on soil medium (Murashige and Skoog salt mixture, 40, 80, and 160 mM of sucrose, glucose, and fructose, 4 μM BA, 0.5 μM NAA, and pH=5.8). The types of carbohydrate and their concentration affected the regeneration. Explant growth measured as fresh weight was maximum on the medium containing 80 mM glucose. The cultures taken from "chilled" shoots developed dormancy and formed bulblets, whereas explants isolated from "non-chilled" shoots were not dormant and produced adventitious shoots only. The glucose- and sucrose-containing medium was superior to the fructose-containing medium in induction of shoots and bulblets. Newly formed bulblets induced on the media with 160 mM sucrose or glucose turned out to be more matured and contained less chlorophyll than those obtained on the one supplemented with fructose as well as with 80 mM sucrose and glucose.

Bach A., Pawłowska B., 1993. Effect of type of carbohydrates in regeneration of Hyacinthus orientalis L. in long-term cultures. Folia Horticulturae V/2: 3-11.