Effect of type of carbohydrates in regeneration of Hyacinthus orientalis L. in long-term cultures
Anna Bach, Bożena Pawłowska
Department of Ornamental Plants
Faculty of Horticulturae, Agricultural University
29 Listopada 54, 31-425 Kraków, Poland
The effects of glucose, sucrose, and fructose on regeneration of the
plantlets on leaf explants of the Hyacinthus orientalis cultivar
Delft's Blue in long-term (2-year-cycle) cultures were compared. The
explants were excised both from cultured shoots chilled at 4°C for 10
weeks and from shoots incubated at 23°C, and then cultivated on soil
medium (Murashige and Skoog salt mixture, 40, 80, and 160 mM of sucrose,
glucose, and fructose, 4 μM BA, 0.5 μM NAA, and pH=5.8). The
types of carbohydrate and their concentration affected the regeneration.
Explant growth measured as fresh weight was maximum on the medium
containing 80 mM glucose. The cultures taken from "chilled"
shoots developed dormancy and formed bulblets, whereas explants isolated
from "non-chilled" shoots were not dormant and produced
adventitious shoots only. The glucose- and sucrose-containing medium was
superior to the fructose-containing medium in induction of shoots and
bulblets. Newly formed bulblets induced on the media with 160 mM sucrose
or glucose turned out to be more matured and contained less chlorophyll
than those obtained on the one supplemented with fructose as well as with
80 mM sucrose and glucose.
Bach A., Pawłowska B., 1993. Effect of type of carbohydrates in regeneration of Hyacinthus
orientalis L. in long-term cultures. Folia Horticulturae V/2: 3-11.