Ethylene production as a biochemical marker for radish seed vigour
Roman Hołubowicz*, Krystyna Tylkowska*, Leonarda Gruchała**
**Department of Seed Science and Technology
**Faculty of Horticulture, Poznań Agricultural University
**Baranowo, 62-081 PrzeĽmierowo, Poland
**Division of Food Concentrate, Institute of Plant Food Technology
**Faculty of Food Technology, Poznań Agricultural University
**Wojska Polskiego 28, 60-662 Poznań, Poland
The studies were conducted on the ethylene production of imbibed and
germinating seed of the radish (Raphanus sativus L.) ‘Saxa’
in order to use this character as a biochemical marker
for seed vigour. The seeds used in the experiments had both high vigour
and germination capacity. Those of low vigour were obtained by subjecting
the given seed lot to accelerated ageing (AA). Determination of ethylene
production was carried out 24, 38, 48, 72, and 96 hours after placing the seeds
on wet blotting paper in Petri dishes. The intensity of ethylene
production was found to depend on seed vigour and the stage of
germination. The highest ethylene production was found after 38 hours
(15.94 nl 50 seed-1 hour-1).
For the seeds subjected to AA the main peak of ethylene production was
recorded 72 hours after placing the seeds on wet blotting paper (12.92 nl
50 seeds-1 hour-1).
Those from the seed lot with a higher ability to produce ethylene, when
sown in the greenhouse, emerged faster than those with lower ability.
Plants from the seeds subjected to AA had shorter aerial parts, a smaller
diameter of the swollen hypocotyl, and lower air-dried weights. The
obtained results showed that estimation of ethylene production could be a
good biochemical indicator of radish seed vigour.
Hołubowicz R., Tylkowska K., Gruchała L., 1993. Ethylene production as a biochemical
marker fo radish seed vigour. Folia Horticulturae V/1: 19-28.