Folia Horticulturae

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Oncidium flexuosum Sims. as a natural host of Odontoglossum ringspot virus (ORSV)

T. Cybularz, T. Kobyłko, K. Miczyński

Department of Botany
Faculty of Horticulture, Agricultural University
29 Listopada 54, 31-425 Kraków, Poland

Odontoglossum ringspot virus (ORSV) was detected in leaves of Oncidium flexuosum Sims. showing severe black necrotic spots or rings ca. 1 cm in diameter. The virus caused only local infection in Chenopodium quinoa Willd., Gomphrena globosa L., Nicotiana glutinosa L., N. tabacum L. cv. Xanthi nc., and Tetragonia expansa Murr.

The physical standard properties regarding the stability of the investigated virus in crude plant sap were as follows: longevity in vitro-over one year, thermal inactivation point: 90°C to 95°C, dilution end-point: 10-3-10-4. In the electron microscope virus particles appeared as solid rods most commonly measuring ca. 280 nm long and 18 nm wide. The serological test (Ouchterlony double diffusion test) gave a positive reaction only with the anti-ORSV diagnostic sera.

Cybularz T., Kobyłko T., Miczyński K., 1992. Oncidium flexuosum Sims. as a natural host of Odontoglossum ringspot virus (ORSV). Folia Horticulturae IV/1: 39-48.