Folia Horticulturae

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Induction of somatic embryogenesis and regeneration of plants of Freesia hybrida cultures

Anna Bach

Institute of Vegetables and Ornamental Plants
Faculty of Horticulture, Agricultural University
29 Listopada 54, 31-425 Kraków, Poland

Embryogenic callus of freesia was initiated in cultures of corm apical meristems of ‘Blue Heaven’ and ‘Yellow Sea’ as well as from mature zygotic embryos of ‘Superfreesia Blue’. The explants formed embryogenic structures (on the average 20%) in the presence of Picloram or 2.4-D, but Picloram was superior to 2.4-D both for induction and maintenance of callus or subsequent regenartion of plants. The differentiation of freesia embryogenic structures by the zygotic embryo explants proved to be better than by the meristem one, but the ability to development of mature embryos was similar for the two primary explants.

Embryo development took place when the embryogenic tissue was transferred to a medium containing a low concentration of auxins. Maturation of somatic embryos was stimulated by cytokinin. An average germination of 5-10% occurred through transfer of somatic embryos to an auxin-free solid medium.

Bach A., 1992. Induction of somatic embryogenesis and regeneration of plants in Freesia hybrida cultures. Folia Horticulturae IV/1: 11-21.