Induction of somatic embryogenesis and regeneration of plants of Freesia hybrida cultures
Institute of Vegetables and Ornamental Plants
Faculty of Horticulture, Agricultural University
29 Listopada 54, 31-425 Kraków, Poland
Embryogenic callus of freesia was initiated in cultures of corm apical
meristems of ‘Blue Heaven’ and ‘Yellow Sea’ as well as from mature zygotic
embryos of ‘Superfreesia Blue’. The explants formed embryogenic structures
(on the average 20%) in the presence of Picloram or 2.4-D, but Picloram
was superior to 2.4-D both for induction and maintenance of callus or
subsequent regenartion of plants. The differentiation of freesia
embryogenic structures by the zygotic embryo explants proved to be better
than by the meristem one, but the ability to development of mature embryos
was similar for the two primary explants.
Embryo development took place when the embryogenic tissue was transferred to a medium containing a low
concentration of auxins. Maturation of somatic embryos was stimulated by
cytokinin. An average germination of 5-10% occurred through transfer of
somatic embryos to an auxin-free solid medium.
Bach A., 1992. Induction of somatic embryogenesis and regeneration of plants in Freesia
hybrida cultures. Folia Horticulturae IV/1: 11-21.