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Micropropagation of the rhododendrons ‘Lee's Dark Purple’ and ‘Nova Zembla’ on three culture media with different levels of growth regulators

Piotr Muras

Institute of Vegetables and Ornamental Plants
Faculty of Horticulture, Agricultural University
29 Listopada 54, 31-425 Kraków, Poland

The tissue culture of rhododendrons ‘Lee's Dark Purple’ and ‘Nova Zembla’ was initiated on Economou and Reads's (1986) medium. 95% of flower peduncles, which were used as initial explants, developed into sterile well-growing cultures. Three media were tested at the multiplication stage, namely Anderson's (1984), Economou and Read's (1986), and the latter modified for the purpose of the present experiment. The last medium (EM modified) proved to be the most suitable for the growth and development of cultures. The high levels of growth regulators had a possitive effect on the rate of shoot propagation. The low content of growth regulators stimulated the elongation of shoots.

Cultures showed considerable variability during the experiment. However, each variety reacted in a different way. Tissue culture of rhododendron initiated by explants of flower peduncles was the source of well-rooting shoots. The population of plants obtained was uniform. Casual deviations observed amounted to 0.11% of the whole population.

Muras P., 1991. Micropopagation of the rhododendrons ‘Lee's Dark Purple’ and ‘Nova Zembla’ on three culture media with different levels of growth regulators. Folia Horticulturae III/2: 65-76.