Folia Horticulturae

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Preliminary identification of a virus infecting Cattleya Waltersiana × Cattleya H.L. Knoles × Cattleya Schönbrunnensis

T. Cybularz, T. Kobyłko, K. Miczyński, Z. Maj

Department of Botany
Faculty of Horticulture, Agricultural University
29 Listopada 54, 31-425 Kraków, Poland

The aim of this work was to identify a pathogenic agent causing necrotic spots on the leaves and colour breaking and deformation of the flowers of Cattleya Waltersiana × Cattleya H.L. Knoles × Cattleya Schönbrunnensis propagated by in vitro cultures in greenhouses of the Botanical Garden in Kraków. Identification tests, carried out on a range of indicator plants, confirmed the viral nature of the disease. The following plant species infected by mechanical inoculation with the sap of diseased Cattleya developed only local infections: Nicotiana tabacum L. cv. Xanthi nc., N. glutinosa L., Chenopodium amaranticolor Costa et Reyn, Ch. quinoa Willd., Tetragonia expansa Murr., and Gomphrena globosa L.

Serological tests gave positive reactions only with antisera prepared for the Odontoglossum ringspot virus (ORSV). The type of symptoms on infected indicator plants, the time of their appearence after infection, the results of serological tests, as well as properties of virus infectivity in extracted sap (TIP - 90-95°C, DEP - 10-3-10-4, LIV - over one year) all show that the diseased Cattleya hybrids were infected with ORSV previously classified as the orchid strain of the tobacco mosaic virus (TMC-0).

Cybularz T., Kobyłko T., Miczyński K., Maj Z., 1990. Preliminary identification of a virus infecting Cattleya Waltersiana × Cattleya H.L. Knoles × Cattleya Schönbrunnensis. Folia Horticulturae II/2: 89-98.