In vitro propagation of brassicas
Department of Plant Breeding and Seed Production
Łobzowska 24, 31-140 Kraków, Poland
Seeds of Brussels sprouts, white cabbage, Chinese cabbage, and Chinese
mustard were sterilized in 4% NaClO and germinated on 0.6% agar. Seedling
tips with cotyledons and hypocotyl segment were cut off and transferred to
media inducing shoot regeneration. In Brussels sprouts 61% of explants
regenerated and in other brassicas over 90%. Shoots isolated from primary
explants were propagated on media with varying content of growth substances. An increased
content of BA stimulated shoot regeneration. The highest coefficients of propagation were obtained
on media with 2.0 mg l-1 BA. The IAA did not exhibit
similar effects. The shoots obtained in cultures were successfully rooted and planted into soil mix.
Pawłowski W., 1990. In vitro propagation of brassicas. Folia Horticulturae II/2: 53-61.