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High efficiency of plant regeneration in epidermal cell layer explant of Hyacinthus orientalis L.

Anna Bach

Institute of Vegetables and Ornamental Plants
Faculty of Horticulture, Agricultural University
29 Listopada 54, 31-425 Kraków, Poland

In the search for an optimal method of hyacinth multiplication in vitro the epidermal and mesophyll cell layers explants isolated from leaves as well as from bulblets tissue have been used. Explants have been taken directly from plantlets and bulblets cv. Delft's Blue and cv. Carnegie regenerated in vitro. Explants isolated from leaves exhibited higher morphogenetic potential to form adventitious shoots in comparison with bulblets, and also the epidermal cell layer explants were more productive than fragment of mesophyll or bulblets tissue. Newly formed advetitious shoots were formed on all wounded edges of the leaf explants. The mean number of shoots and/or leaves per epidermal explant varied between 9.0-11.3. The epidermal cell layer explants of leaves proved beneficial for the multiplication stage and could be used in a new effective method of a year-round cycle of hyacinth micropropagation.

Bach A., 1989. High efficiency of plant regeneration in epidermal cell layer explant of Hyacinthus orientalis L. Folia Horticulturae I/1: 45-53.