High efficiency of plant regeneration in epidermal cell layer explant of
Hyacinthus orientalis L.
Institute of Vegetables and Ornamental Plants
Faculty of Horticulture, Agricultural University
29 Listopada 54, 31-425 Kraków, Poland
In the search for an optimal method of hyacinth multiplication in vitro
the epidermal and mesophyll cell layers explants isolated from leaves as
well as from bulblets tissue have been used. Explants have been taken
directly from plantlets and bulblets cv. Delft's Blue and cv. Carnegie
regenerated in vitro. Explants isolated from leaves exhibited higher
morphogenetic potential to form adventitious shoots in comparison with
bulblets, and also the epidermal cell layer explants were more productive
than fragment of mesophyll or bulblets tissue. Newly formed advetitious
shoots were formed on all wounded edges of the leaf explants. The mean
number of shoots and/or leaves per epidermal explant varied between
9.0-11.3. The epidermal cell layer explants of leaves proved beneficial
for the multiplication stage and could be used in a new effective method
of a year-round cycle of hyacinth micropropagation.
Bach A., 1989. High efficiency of plant regeneration in epidermal cell layer explant of Hyacinthus orientalis L.
Folia Horticulturae I/1: 45-53.